Blot Western: Perbedaan antara revisi

[[Berkas:Western Blot.png|thumbjmpl|Sebuah western blot.]]

'''Blot Western''' adalah sebuah metode untuk mendeteksi [[protein]] pada sampel jaringan. Imunoblot menggunakan [[elektroforesis gel]] untuk memisahkan protein asli atau perubahan oleh jarak polipeptida atau oleh struktur 3-D protein. Protein tersebut dikirim ke membran, di mana mereka dideteksi menggunakan [[antibodi]] untuk menargetkan protein.

* Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", ''Proc Natl Acad Sci U S A'', 1979 Jul;76(7):3116-20. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=91164 PMID: 91164] [http://www.pnas.org/cgi/content/abstract/76/7/3116 abstract] {{Webarchive|url=https://web.archive.org/web/20080323132129/http://www.pnas.org/cgi/content/abstract/76/7/3116 |date=2008-03-23 }}

* Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" ''Proc Natl Acad Sci U S A'', 1979 Sep;76(9):4350-4. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=388439 PMID: 388439] [http://www.pnas.org/cgi/content/abstract/76/9/4350 abstract] {{Webarchive|url=https://web.archive.org/web/20080317111721/http://www.pnas.org/cgi/content/abstract/76/9/4350 |date=2008-03-17 }}

=== Pranala luar ===

* [http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System (a protocol)] {{Webarchive|url=https://web.archive.org/web/20100420005952/http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php |date=2010-04-20 }}

* [http://www.prosci-inc.com/Western-Blot-Protocol.html Western blot protocol including example buffers and reprobing procedure] {{Webarchive|url=https://web.archive.org/web/20071114213552/http://www.prosci-inc.com/Western-Blot-Protocol.html |date=2007-11-14 }}

[[Kategori:TeknikMetode laboratorium]]

[[Kategori:Biologi molekularmolekuler]]