Mikroskop cahaya: Perbedaan antara revisi

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== Tipe ==
[[Berkas:Microscope simple diagram.png|thumb|right|150px|Diagram sebuah mikroskop sederhana]]
Terdapat dua tipe mikroskop cahaya, yakni mikroskop sederhana dan mikroskop majemuk.<ref>{{Cite book|last=Harijati|first=Nunung|last2=Samino|first2=Setijono|last3=Indriyani|first3=Serafinah|last4=Soewondo|first4=Aris|date=Oktober 2017|url=https://books.google.co.id/books?id=RxRTDwAAQBAJ&pg=PA3|title=Mikroteknik Dasar|location=Malang|publisher=UB Press|isbn=|pages=3-5|url-status=live}}</ref> Mikroskop sederhana menggunakan [[daya optis]] dari lensa tunggal atau pasanganset lensa untuk menghasilkan perbesaran suatu objek. Mikroskop sederhana umumnya dijual di pasaran sebagai [[mikroskop digital]] dengan harga yang murah. Sementara itu, mikroskop majemuk menggunakan sebuah sistem lensa untuk menghasilkan perbesaran yang lebih besar. Pada mikroskop majemuk, sepasang lensa digunakan untuk memperbesar gambar yang telah diperbesar oleh lensa lainnya. MikroskopSebagian besar mikroskop yang digunakan untuk [[penelitian]] yang umum digunakan saat ini merupakan mikroskop majemuk. Mikroskop majemuk dapat dibagi lagi menjadi beberapa tipe lain berdasarkan fungsi, konfigurasi optis, dan harganya.
 
 
=== Mikroskop sederhana ===
Mikroskop sederhana menggunakan lensa tunggal atau sepasang lensa untuk menghasilkan perbesaran objek. Bayangan yang dihasilkan oleh mikroskop sederhana bersifat maya, tegak, dan diperbesar.<ref>{{cite book|author=Trisha Knowledge Systems|title=The IIT Foundation Series - Physics Class 8, 2/e|url=https://books.google.com/books?id=NKh9dQKnTdEC&pg=PA213|publisher=Pearson Education India|isbn=978-81-317-6147-2|page=213}}</ref> Penggunaan lensa cembung tunggal seperti ini dapat ditemukan pada alat perbesaran seperti [[kaca pembesar]], [[lup]], dan lensa okuler.
 
<!--=== Mikroskop majemuk===
[[Berkas:Microscope compound diagram.png|thumb|left|150px|Diagram mikroskop majemuk]]
AMikroskop compoundmajemuk microscopemenggunakan usessebuah alensa lensdi closedekat to the object being viewed to collect lightobjek (called the [[Objectivelensa (optics)|objectiveobjektif]] lens) whichuntuk focuses amemfokuskan [[realbayangan imagenyata]] of thedari objectobjek insidedi thedalam microscopemikroskop (imagelihat 1gambar). ThatBayangan imageobjek iskemudian thendiperbesar magnifiedmenggunakan bysatu aatau secondbeberapa lenslensa orlainnya groupdi ofdekat lensespengamat (called the [[eyepiecelensa objektif]]) thatyang givesmemberikan thebayangan viewerdiperbesar ankepada enlargedpengamat. invertedBayangan virtualyang imagedihasilkan ofoleh themiskroskop objectmajemuk (imagebersifat 2)nyata, terbaik, dan diperbesar.<ref name=Watt>{{cite book|author=Ian M. Watt|title=The Principles and Practice of Electron Microscopy|url=https://books.google.com/books?id=Y6-sE4gUX-QC&pg=PA6|year=1997|publisher=Cambridge University Press|isbn=978-0-521-43591-8|page=6}}</ref> ThePenggunaan usekombinasi oflensa aobjektif compounddan objective/eyepieceokuler combinationmenghasilkan allowsbayangan foryang muchlebih higher magnificationbesar. CommonLensa compoundpada microscopesmikroskop oftenmajemuk featurejuga exchangeableseringkali objectivedapat lenses,diganti allowinguntuk themendapatkan userperbesaran toyang quickly adjust the magnificationdiinginkan.<ref name=Watt/> A compound microscope also enables more advanced illumination setups, such as [[phase contrast]].-->
 
<!--===Varian mikroskop lainnya===
Terdapat banyak varian mikroskop majemuk yang dibedakan berdasarkan fungsinya. Beberapa varian memiliki bentuk fisik yang berbeda untuk digunakan pada fungsi-fungsi tertentu:
 
* [[Mikroskop stereo]], a low-powered microscope which provides a stereoscopic view of the sample, commonly used for dissection.
* [[Comparison microscope]], which has two separate light paths allowing direct comparison of two samples via one image in each eye.
* [[Inverted microscope]], for studying samples from below; useful for cell cultures in liquid, or for metallography.
* Fiber optic connector inspection microscope, designed for connector end-face inspection
* [[Traveling microscope]], for studying samples of high [[optical resolution]].
 
Other microscope variants are designed for different illumination techniques:
* [[Petrographic microscope]], whose design usually includes a polarizing filter, rotating stage and gypsum plate to facilitate the study of minerals or other crystalline materials whose optical properties can vary with orientation.
* [[Polarizing microscope]], similar to the petrographic microscope.
* [[Phase-contrast microscope]], which applies the phase contrast illumination method.
* [[Epifluorescence microscope]], designed for analysis of samples which include fluorophores.
* [[Confocal microscope]], a widely used variant of epifluorescent illumination which uses a scanning laser to illuminate a sample for fluorescence.
*[[Two-photon excitation microscopy|Two-photon microscope]], used to image fluorescence deeper in scattering media and reduce photobleaching, especially in living samples.
* Student microscope – an often low-power microscope with simplified controls and sometimes low quality optics designed for school use or as a starter instrument for children.<ref>{{cite web|url=http://www.well.ox.ac.uk/_asset/file/buying-a-cheap-microscope-for-home.pdf|title=Buying a cheap microscope for home use|access-date=5 November 2015|publisher=Oxford University.|url-status=live|archiveurl=https://web.archive.org/web/20160305042314/http://www.well.ox.ac.uk/_asset/file/buying-a-cheap-microscope-for-home.pdf|archivedate=5 March 2016}}</ref>
* [[Ultramicroscope]], an adapted light microscope that uses [[light scattering]] to allow viewing of tiny particles whose diameter is below or near the wavelength of visible light (around 500 nanometers); mostly obsolete since the advent of [[electron microscope]]s
*[[Tip-enhanced Raman spectroscopy|Tip-enhanced Raman microscope]], is a variant of optical microscope based on [[tip-enhanced Raman spectroscopy]], without traditional wavelength-based resolution limits.<ref>{{Cite journal|last=Kumar|first=Naresh|last2=Weckhuysen|first2=Bert M.|last3=Wain|first3=Andrew J.|last4=Pollard|first4=Andrew J.|date=April 2019|title=Nanoscale chemical imaging using tip-enhanced Raman spectroscopy|journal=Nature Protocols|volume=14|issue=4|pages=1169–1193|doi=10.1038/s41596-019-0132-z|pmid=30911174|issn=1750-2799|doi-access=free}}</ref><ref>{{Cite journal|last=Lee|first=Joonhee|last2=Crampton|first2=Kevin T.|last3=Tallarida|first3=Nicholas|last4=Apkarian|first4=V. Ara|date=April 2019|title=Visualizing vibrational normal modes of a single molecule with atomically confined light|journal=Nature|volume=568|issue=7750|pages=78–82|doi=10.1038/s41586-019-1059-9|pmid=30944493|issn=1476-4687}}</ref> This microscope primarily realized on the [[Scanning probe microscopy|scanning-probe microscope]] platforms using all optical tools.-->
 
== Jenis lensa ==