Archaeol: Perbedaan antara revisi

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Dalam rangka menganalisis kandungan archaeol, lipid biasanya harus diekstraksi melalui prosedur Bligh-Dyer tradisional.<ref>{{Cite journal|last=Bligh|first=E. G.|last2=Dyer|first2=W. J.|date=1959-08-01|title=A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION|url=http://www.nrcresearchpress.com/doi/10.1139/o59-099|journal=Canadian Journal of Biochemistry and Physiology|language=en|volume=37|issue=8|pages=911–917|doi=10.1139/o59-099|issn=0576-5544}}</ref> Prosedur diikuti oleh fraksinasi (dengan kromatografi kolom atau lapisan tipis) dan derivatisasi. Proses serupa yang melibatkan acid Bligh dan ekstraksi Dyer, perlakuan asam dan derivatisasi, juga diusulkan oleh kelompok peneliti seperti Kazuhiro Demizu dkk.<ref name=":3">{{Cite journal|last=Demizu|first=Kazuhiro|last2=Ohtsubo|first2=Sadami|last3=Kohno|first3=Shuhei|last4=Miura|first4=Isao|last5=Nishihara|first5=Masateru|last6=Koga|first6=Yosuke|date=1992|title=Quantitative determination of methanogenic cells based on analysis of ether-linked glycerolipids by high-performance liquid chromatography|url=https://linkinghub.elsevier.com/retrieve/pii/0922338X92905537|journal=Journal of Fermentation and Bioengineering|language=en|volume=73|issue=2|pages=135–139|doi=10.1016/0922-338X(92)90553-7}}</ref> dan Sadami Ohtsubo et al.<ref name=":4">{{Cite journal|last=Ohtsubo|first=Sadami|last2=Mitsuyoshi Kanno|last3=Hiroyoshi Miyahara|last4=Shuhei Kohno|last5=Yosuke Koga|last6=Isao Miura|date=1993-05|title=A sensitive method for quantification of aceticlastic methanogens and estimation of total methanogenic cells in natural environments based on an analysis of ether-linked glycerolipids|url=https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.1993.tb00015.x|journal=FEMS Microbiology Ecology|language=en|volume=12|issue=1|pages=39–50|doi=10.1111/j.1574-6941.1993.tb00015.x}}</ref>
 
Teknologi seperti kromatografi biasanya digunakan ntuk mampu menentukan konsentrasi archaeol dalam sebuah sampel. Salah satunya termasuk high-performance liquid chromatography (HPLC),<ref name=":3" /><ref name=":4" /><ref>{{Cite journal|last=Martz|first=Robert F.|last2=Sebacher|first2=Daniel I.|last3=White|first3=David C.|date=1983-02|title=Biomass measurement of methane forming bacteria in environmental samples|url=https://linkinghub.elsevier.com/retrieve/pii/0167701283900076|journal=Journal of Microbiological Methods|language=en|volume=1|issue=1|pages=53–61|doi=10.1016/0167-7012(83)90007-6}}</ref> Kromatografi gas (GC),<ref>{{Cite journal|last=Smith|first=G.C.|last2=Floodgate|first2=G.D.|date=1992-10|title=A chemical method for estimating methanogenic biomass|url=https://linkinghub.elsevier.com/retrieve/pii/027843439290078X|journal=Continental Shelf Research|language=en|volume=12|issue=10|pages=1187–1196|doi=10.1016/0278-4343(92)90078-X}}</ref> dan supercritical fluid chromatography (SFC).<ref>{{Cite journal|last=Holzer|first=Gunther U.|last2=Kelly|first2=Patrick J.|last3=Jones|first3=William J.|date=1988-07|title=Analysis of lipids from a hydrothermal vent methanogen and associated vent sediment by supercritical fluid chromatography|url=https://linkinghub.elsevier.com/retrieve/pii/0167701288900176|journal=Journal of Microbiological Methods|language=en|volume=8|issue=3|pages=161–173|doi=10.1016/0167-7012(88)90017-6}}</ref><ref>{{Cite book|last=King|first=Jerry|date=2002-01-22|url=http://www.crcnetbase.com/doi/10.1201/9780203908198.ch34|title=Lipid Biotechnology|publisher=CRC Press|isbn=978-0-8247-0619-7|editor-last=Gardner|editor-first=Harold|language=en|doi=10.1201/9780203908198.ch34|editor-last2=Min Kuo|editor-first2=Tsung}}{{Pranala mati|date=Februari 2021 |bot=InternetArchiveBot |fix-attempted=yes }}</ref> [[Spektrometri massa]] (MS) sering digunakan untuk membantu identifikasi pada banyak kasus.
 
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