Pelatih kepala: [[Jaroslav Šilhavý]]
[[Tim nasional sepak bola Ceko|Ceko]] mengumumkan 25 pemain dari skuadskuat finalakhir mereka pada 25 Mei 2021.<ref>{{cite news |url=https://repre.fotbal.cz/ceska-reprezentace-oznamila-nominaci-na-uefa-euro-2020/a14226 |title=Česká reprezentace oznámila nominaci na UEFA EURO 2020 |trans-title=The Czech national team announced the squad for UEFA Euro 2020 |work=[[Football Association of the Czech Republic]] |date=25 May 2021 |access-date=25 May 2021 |language=cs}}</ref> [[Michal Sadílek]] diumumkan sebagai pemain terakhir dalam skuad pada 27 Mei, setelah konfirmasi larangan bermain sepuluh pertandingan kepada [[Ondřej Kúdela]].<ref>{{cite news |url=https://repre.fotbal.cz/reprezentace-je-kompletni-nominaci-na-euro-2020-doplnil-sadilek/a14243 |title=Reprezentace je kompletní: nominaci na EURO 2020 doplnil Sadílek |trans-title=The team is complete: Sadílek added to the squad for Euro 2020 |work=[[Football Association of the Czech Republic]] |date=27 May 2021 |access-date=27 May 2021 |language=cs}}</ref> [[Jiří Pavlenka]] digantikan oleh [[Tomáš Koubek]] karena cedera pada 12 Juni.<ref>{{cite news |url=https://www.fotbal.cz/euro2020/zmena-v-nominaci-na-euro-zraneneho-pavlenku-nahradi-koubek/a14367 |title=Změna v nominaci na EURO: zraněného Pavlenku nahradí Koubek |trans-title=Change in Euro squad: the injured Pavlenka will be replaced by Koubek |work=[[Football Association of the Czech Republic]] |date=12 June 2021 |access-date=12 June 2021 |language=cs}}</ref>
{{nat fs g start}}
{{nat fs g player|no=1|pos=GK|name=[[Tomáš Vaclík]]|sortname=Vaclik, Tomas|age={{birth date and age2|df=y|2021|6|11|1989|3|29}}|caps=3637|goals=0|club=[[Sevilla FC|Sevilla]]|clubnat=ESP}}
{{nat fs g player|no=2|pos=DF|name=[[Pavel Kadeřábek]]|sortname=Kaderabek, Pavel|age={{birth date and age2|df=y|2021|6|11|1992|4|25}}|caps=47|goals=3|club=[[TSG 1899 Hoffenheim|1899 Hoffenheim]]|clubnat=GER}}
{{nat fs g player|no=3|pos=DF|name=[[Ondřej Čelůstka]]|sortname=Celustka, Ondrej|age={{birth date and age2|df=y|2021|6|11|1989|6|18}}|caps=2426|goals=23|club=[[AC Sparta PraguePraha|Sparta PraguePraha]]|clubnat=CZE}}
{{nat fs g player|no=4|pos=DF|name=[[DavidJakub ZimaBrabec]]|sortname=ZimaBrabec, DavidJakub|age={{birth date and age2|df=y|2021|6|11|2000|111992|8|6}}|caps=121|goals=01|club=[[SKFC SlaviaViktoria PraguePlzeň|SlaviaViktoria PraguePlzeň]]|clubnat=CZE}}
{{nat fs g player|no=5|pos=DF|name=[[Vladimír Coufal]]|sortname=Coufal, Vladimir|age={{birth date and age2|df=y|2021|6|11|1992|8|22}}|caps=1416|goals=1|club=[[West Ham United F.C.|West Ham United]]|clubnat=ENG}}
{{nat fs g player|no=6|pos=DF|name=[[Tomáš Kalas]]|sortname=Kalas, Tomas|age={{birth date and age2|df=y|2021|6|11|1993|5|15}}|caps=2223|goals=2|club=[[Bristol City F.C.|Bristol City]]|clubnat=ENG}}
{{nat fs g player|no=7|pos=MF|name=[[Antonín Barák]]|sortname=Barak, Antonin|age={{birth date and age2|df=y|2021|6|11|1994|12|3}}|caps=1820|goals=6|club=[[Hellas Verona F.C.|Hellas Verona]]|clubnat=ITA}}
{{nat fs g player|no=8|pos=MF|name=[[Vladimír Darida]] {{kapten}}|sortname=Darida, Vladimir|other=[[Captain (association football)|captain]]|age={{birth date and age2|df=y|2021|6|11|1990|8|8}}|caps=7072|goals=8|club=[[Hertha BSC]]|clubnat=GER}}
{{nat fs g player|no=9|pos=FWDF|name=[[PatrikTomáš SchickHoleš]]|sortname=SchickHoles, PatrikTomas|age={{birth date and age2|df=y|2021|6|11|19961993|13|2431}}|caps=248|goals=101|club=[[BayerSK 04Slavia LeverkusenPraha|BayerSlavia LeverkusenPraha]]|clubnat=GERCZE}}
{{nat fs g player|no=10|pos=FW|name=[[TomášPatrik PekhartSchick]]|sortname=PekhartSchick, TomasPatrik|age={{birth date and age2|df=y|2021|6|11|19891996|51|2624}}|caps=2126|goals=211|club=[[LegiaBayer 04 Leverkusen|Bayer WarsawLeverkusen]]|clubnat=POLGER}}
{{nat fs g player|no=11|pos=FW|name=[[Michael Krmenčík]]|sortname=Krmencik, Michael|age={{birth date and age2|df=y|2021|6|11|1993|3|15}}|caps=2829|goals=9|club=[[PAOK FC|PAOK]]|clubnat=GRE}}
{{nat fs g player|no=12|pos=MF|name=[[Lukáš Masopust]]|sortname=Masopust, Lukas|age={{birth date and age2|df=y|2021|6|11|1993|2|12}}|caps=2022|goals=12|club=[[SK Slavia PraguePraha|Slavia PraguePraha]]|clubnat=CZE}}
{{nat fs g player|no=13|pos=MF|name=[[Petr Ševčík]]|sortname=Sevcik, Petr|age={{birth date and age2|df=y|2021|6|11|1994|5|4}}|caps=67|goals=0|club=[[SK Slavia PraguePraha|Slavia PraguePraha]]|clubnat=CZE}}
{{nat fs g player|no=14|pos=MF|name=[[Jakub Jankto]]|sortname=Jankto, Jakub|age={{birth date and age2|df=y|2021|6|11|1996|1|19}}|caps=3335|goals=4|club=[[U.C. Sampdoria|Sampdoria]]|clubnat=ITA}}
{{nat fs g player|no=15|pos=MF|name=[[Tomáš Souček]]|sortname=Soucek, Tomas|age={{birth date and age2|df=y|2021|6|11|1995|2|27}}|caps=3335|goals=7|club=[[West Ham United F.C.|West Ham United]]|clubnat=ENG}}
{{nat fs g player|no=16|pos=GK|name=[[Aleš Mandous]]|sortname=Mandous, Ales|age={{birth date and age2|df=y|2021|6|11|1992|4|21}}|caps=1|goals=0|club=[[SK Sigma Olomouc|Sigma Olomouc]]|clubnat=CZE}}
{{nat fs g player|no=17|pos=DF|name=[[AlešDavid MatějůZima]]|sortname=MatejuZima, AlesDavid|age={{birth date and age2|df=y|2021|6|11|19962000|611|38}}|caps=42|goals=0|club=[[BresciaSK CalcioSlavia Praha|BresciaSlavia Praha]]|clubnat=ITACZE}}
{{nat fs g player|no=18|pos=DF|name=[[Jan Bořil]]|sortname=Boril, Jan|age={{birth date and age2|df=y|2021|6|11|1991|1|11}}|caps=2123|goals=0|club=[[SK Slavia PraguePraha|Slavia PraguePraha]]|clubnat=CZE}}
{{nat fs g player|no=19|pos=DFFW|name=[[TomášAdam HolešHložek]]|sortname=HolesHlozek, TomasAdam|age={{birth date and age2|df=y|2021|6|11|19932002|37|3125}}|caps=73|goals=10|club=[[SKAC SlaviaSparta PraguePraha|SlaviaSparta PraguePraha]]|clubnat=CZE}}
{{nat fs g player|no=20|pos=FW|name=[[Matěj Vydra]]|sortname=Vydra, Matej|age={{birth date and age2|df=y|2021|6|11|1992|5|1}}|caps=3536|goals=6|club=[[Burnley F.C.|Burnley]]|clubnat=ENG}}
{{nat fs g player|no=21|pos=MF|name=[[Alex Král]]|sortname=Kral, Alex|age={{birth date and age2|df=y|2021|6|11|1998|5|19}}|caps=1618|goals=2|club=[[FC Spartak MoscowMoskwa|Spartak MoscowMoskwa]]|clubnat=RUS}}
{{nat fs g player|no=22|pos=FWDF|name=[[AdamAleš HložekMatějů]]|sortname=HlozekMateju, AdamAles|age={{birth date and age2|df=y|2021|6|11|20021996|76|253}}|caps=24|goals=0|club=[[ACBrescia Sparta PragueCalcio|Sparta PragueBrescia]]|clubnat=CZEITA}}
{{nat fs g player|no=23|pos=GK|name=[[JiříTomáš PavlenkaKoubek]]|sortname=PavlenkaKoubek, JiriTomas|age={{birth date and age2|df=y|2021|6|11|1992|48|1426}}|caps=1311|goals=0|club=[[SV Werder Bremen|WerderFC BremenAugsburg]]|clubnat=GER}}
{{nat fs g player|no=24|pos=DFFW|name=[[JakubTomáš BrabecPekhart]]|sortname=BrabecPekhart, JakubTomas|age={{birth date and age2|df=y|2021|6|11|19921989|85|626}}|caps=2022|goals=12|club=[[FC Viktoria Plzeň|ViktoriaLegia PlzeňWarsawa]]|clubnat=CZEPOL}}
{{nat fs g player|no=25|pos=FWMF|name=[[Jakub Pešek]]|sortname=Pesek, Jakub|age={{birth date and age2|df=y|2021|6|11|1993|6|24}}|caps=12|goals=1|club=[[FC Slovan Liberec|Slovan Liberec]]|clubnat=CZE}}
{{nat fs g player|no=26|pos=MF|name=[[Michal Sadílek]]|sortname=Sadilek, Michal|age={{birth date and age2|df=y|2021|6|11|1999|5|31}}|caps=01|goals=0|club=[[FC Slovan Liberec|Slovan Liberec]]|clubnat=CZE}}
{{nat fs end}}
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